The Effect of The Topical Application of Eurycoma Longifolia Jack (TA) Root Extract Hydrogel on Vascular Endothelial Growth Factor (VEGF) Expression During Wound Healing in vivo Excisional Wound Model

INTRODUCTION: Medicinal plants are known for their positive impacts on wound healing by stimulating angiogenesis in the skin. Eurycoma longifolia Jack locally as Tongkat Ali (TA), is a medicinal plant characterised anti-inflammatory and antioxidant effects. MATERIALS AND METHODS: Excisional (15mm × 15mm) diameter (2mm) depth was created at back of 20 male Sprague Dawley rats incising marked skin with sterilized surgical scalpel blade then excised scissors toothed forceps. The wounded were divided into 4 groups, each group contained 5 (n=5). Experimental groups formed follows: untreated (-ve) control, Hydrocyn® aqua gel (+ve) vehicle hydrogel, TA hydrogel. All treatments applied twice daily days starting first day (wounding day). On Day post-wounding, granulation tissue harvested from all evaluated immunohistochemistry assay Vascular endothelial growth factor (VEGF) expression. RESULTS: VEGF expression rat’s treated hydrogel increased significantly compared to that control p-values 0.040 0.029, respectively. Although there no significant difference group, our study showed higher VEGF. CONCLUSION: Our topical application skin, which an essential healing. Thus, great potential be effective wound-healing agent managing cutaneous wounds.